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KMID : 1094720110160020360
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Biotechnology and Bioprocess Engineering
2011 Volume.16 No. 2 p.360 ~ p.365
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Purification and biochemical characterization of recombinant human H-ferritins from Saccharomyces cerevisiae
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Seo Hyang-Yim
Kim Kyung-Suk
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Abstract
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Recombinant human H-ferritins produced from Saccharomyces cerevisiae were purified and their molecular properties were characterized. Electrophoresis of the recombinant H-ferritins showed revealed bands with mobilities similar to those of the H-ferritins produced by Escherichia coli. The pI of H-ferritins from yeast was more basic than that of H-ferritins produced by E. coli. When the cells were treated with tunicamycin, the pI of H-ferritins was driven to a sharp band with the pI range similar to that of those produced by E. coli, implying that the H-ferritins from yeast are glycosylated. The iron uptake of H-ferritins was rapid in the initial stage, with a slight reduction when compared to ferritins from E. coli. Recombinant ferritins are commonly used during synthesis of inorganic nanoparticles in their cores for chemical and industrial purposes. Transmission electron microscopy revealed that the reconstitution yield and size distribution of the core minerals were affected depending on the protein shells. The H-ferritins with higher reconstitution yields (64.4%) showed slightly larger sizes (mean 6.52 nm) with narrower size distribution.
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KEYWORD
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H-ferritin, iron kinetics, core reconstitution, glycosylation, yeast, E. coli
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